Rapid Scanning Structure–Activity Relationships in Combinatorial Data Sets: Identification of Activity Switches

Rapid mapping of protein functional epitopes by combinatorial alanine scanning.

A combinatorial alanine-scanning strategy was used to determine simultaneously the functional contributions of 19 side chains buried at the interface between human growth hormone and the extracellular domain of its receptor. A phage-displayed protein library was constructed in which the 19 side chains were preferentially allowed to vary only as the wild type or alanine. The library pool was sub...

comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.

heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...

Generating Realistic Data Sets for Combinatorial Auctions

A convex combinatorial property of compact sets in the plane and its roots in lattice theory

K. Adaricheva and M. Bolat have recently proved that if $,mathcal U_0$ and $,mathcal U_1$ are circles in a triangle with vertices $A_0,A_1,A_2$, then there exist $jin {0,1,2}$ and $kin{0,1}$ such that $,mathcal U_{1-k}$ is included in the convex hull of $,mathcal U_kcup({A_0,A_1, A_2}setminus{A_j})$. One could say disks instead of circles.Here we prove the existence of such a $j$ and $k$ ...

A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display

There is growing interest in the fast and robust engineering of protein pH-sensitivity that aims to reduce binding at acidic pH, compared to neutral pH. Here, we describe a novel strategy for the incorporation of pH-sensitive antigen binding functions into antibody variable domains using combinatorial histidine scanning libraries and yeast surface display. The strategy allows simultaneous scree...